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Negative Test - List of Manufacturers, Suppliers, Companies and Products

Negative Test Product List

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Safety testing of cells and culture supernatants: Mycoplasma detection test

We will conduct a mycoplasma negative test on the sample using real-time PCR or the MycoAlert method.

We will conduct a mycoplasma detection test for samples such as culture supernatants. Our company offers a choice between two methods: real-time PCR or MycoAlert. <MycoAlert Method> This measurement method utilizes the activity of enzymes from mycoplasmas, which are present in the majority of 180 species of mycoplasma and not found in eukaryotic cells. <Real-time PCR Method (TaKaRa Mycoplasma qPCR Detection Kit)> Using the real-time PCR method, we can detect seven species of mycoplasma genes specified by official methods such as the Japanese Pharmacopoeia in about one hour. Additionally, we use a validated method as indicated in the "Mycoplasma Detection Test C. Nucleic Acid Amplification Test (NAT)" of the 18th Revised Japanese Pharmacopoeia reference information for cell substrates used in the manufacture of biotechnology-derived pharmaceuticals/bio-origin pharmaceuticals. Please feel free to inquire about acceptable samples, required sample volumes, prices, etc.

  • Other analysis and evaluation services

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Quality Test "Mycoplasma Negative Test"

Designed to detect over 90 types of mycoplasma! Testing using real-time PCR method.

Our company offers "Mycoplasma Detection Testing." Due to its size, mycoplasma can pass through sterilizing filters, and many antibiotics are ineffective against it, making it easy to contaminate cell culture media. Additionally, even when it contaminates and proliferates in cell culture media, it is difficult to detect visually, necessitating special management. We conduct mycoplasma detection testing using a real-time PCR method designed to detect over 90 types of mycoplasma. We also offer testing to confirm the suitability of measurements by adding a known quantity of mycoplasma DNA to the sample. [Features] - Mycoplasma can pass through sterilizing filters due to its size. - It is easy to contaminate cell culture media because it is resistant to antibiotics. - Special management is required because it is difficult to visually detect even when it contaminates and proliferates in cell culture media. - Real-time PCR method designed to detect over 90 types of mycoplasma. - We also conduct tests to confirm measurement suitability by adding a known quantity of mycoplasma DNA to the sample. *For more details, please refer to the related links or feel free to contact us.*

  • Contract measurement

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"Safety testing of cells and cell-derived products" Virus inactivation test

We will conduct a virus negation test using real-time PCR.

We measure nine human viruses (HIV1, HIV2, HCV, HBV, HTLV, ParvoB19, WNV, EBV, CMV) recommended for testing to ensure the quality and safety of human (same species) somatic stem cell processing pharmaceuticals using real-time PCR. For HIV, HIV1, HIV2, HCV, HBV, HTLV1, and HTLV2, measurements can be performed individually. Testing kit: Virus Test Kit (manufactured by Takara Bio) Please feel free to consult us regarding acceptable specimens, required specimen volumes, prices, etc.

  • Other analysis and evaluation services

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